When the PY20 anti-phosphotyrosine monoclonal antibody was used as the probe, no tyrosine-phosphorylated CA125 was detected in cell lysates. Our posters are produced on acid-free papers using archival inks to guarantee that they. I wish I could tell my human how much Ive loved him, after the twelve years weve been together.
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Although CA125 could be immunoprecipitated from WISH cell media incubated with -orthophosphate in the presence or absence of vanadate as detected by Western blotting, autoradiographic analysis of the Western blots revealed no -labelled CA125 co-migrating with the 200-kD plus molecule detected by M11. Listen online to Aikagi - I wish I was a Human and find out more about its history, critical reception, and meaning. I wish I could tell Al eleven, when my human brings him home, to take care of him in his moods, when hes happy, when hes sad, when hes so upset that he cant even breathe. CA125 immunoprecipitated from vanadate-treated cells showed a marked increase in cell-associated CA125 phosphorylation. Immunoprecipitates from cell lysates demonstrated that a phosphoprotein of > 200 kD was isolated and immunoreacted with both the OC125 and M11 anti-CA125 monoclonal antibodies by Western blotting. M11 immunoprecipitation of CA125 from cells labelled with -orthophosphate was analyzed by SDS-PAGE and autoradiography.
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Immunohistochemistry of vanadate-treated cells resulted in a substantial increase in not only cytoplasmic tyrosine phosphoproteins but also in membrane-associated CA125 when stained with the PY20 anti-phosphotyrosine and M11 anti-CA125 monoclonal antibodies, respectively. Exposure of vanadate-treated cells to epidermal growth factor further enhanced this secretory activity.
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WISH cells treated with a tyrosine phosphatase inhibitor, vanadate/ H2O2, resulted in increased levels of CA125 secretion. It is also indicated that regulation of CA125 secretion requires protein(s) tyrosine phosphorylation. The Wish Human Hair wig by Ellen Wille Pure Power Collection is a natural looking ladies style has been made using the finest Remy which is 100 processed. Evidence is presented suggesting that CA125 is a serine and/or threonine phosphoprotein and that its secretion from the human amnion WISH cell line is closely linked to its phosphorylation.